Batch Verification Report
Batch verification and analytical summary for BPC-157 + TB-500 Fragment Blend (dual-component research peptide blend).
TB-500 Fragment (Thymosin Beta-4 Fragment 17–23)
Filler-free lyophilised peptide powder · No mannitol or bulking agents
TB-500 Frag Lot: P251226-A033 · Mfg: 05 Jan 2026 · Retest: 04 Jan 2028
Amount: As specified on Aventris product label
Analytical Results
The following analytical data was obtained from manufacturer testing of the source batches. Aventris has independently verified this data against our quality specifications. This Batch Verification Report summarises our review findings for BPC-157 and TB-500 Fragment.
| Test | Method | Specification | Result |
|---|---|---|---|
| Appearance | Visual | White powder | Conforms |
| Identity (molecular weight) | ESI-MS | 1419.75 ± 1.0 Da | Conforms |
| Purity | HPLC | ≥ 98.0% | 99.57% |
| Peptide content | Nitrogen analysis (N%) | ≥ 80.0% | 96.21% |
| Water content | Karl Fischer | ≤ 10.0% | 2.75% |
| Acetate content | HPLC | < 12.0% | 0.54% |
| Residual solvents – Acetonitrile | GC | NMT 410 ppm | Not detected |
| Residual solvents – Methanol | GC | NMT 3000 ppm | Not detected |
| Bacterial endotoxins | Pharmacopeial method | < 10 EU/mg | Conforms |
| Total aerobic microbial count | Plate count | ≤ 1000 cfu/g | Conforms |
| Total yeast & mold count | Plate count | ≤ 200 cfu/g | Conforms |
| Assay (HPLC) | HPLC | 95.0 – 105.0% (anhydrous, salt-free basis) | 99.50% |
| Test | Method | Specification | Result |
|---|---|---|---|
| Appearance | Visual | White powder | Conforms |
| Solubility (water) | Solubility check | Soluble in water | Conforms |
| Identity (molecular weight) | ESI-MS | 889.01 ± 1.0 Da | Conforms |
| Purity | HPLC | ≥ 99.0% | 99.70% |
| Peptide content | Nitrogen analysis (N%) | ≥ 80.0% | 89.54% |
| Water content | Karl Fischer | ≤ 10.0% | 2.15% |
| Acetate content | HPLC | < 12.0% | 2.96% |
| TFA content | HPLC | ≤ 0.5% | Not detected |
| Residual solvents – Acetonitrile | GC | NMT 410 ppm | Not detected |
| Residual solvents – Ethyl ether | GC | NMT 5000 ppm | Not detected |
| Residual solvents – Dichloromethane | GC | NMT 600 ppm | Not detected |
| Residual solvents – Pyridine | GC | NMT 200 ppm | Not detected |
| Residual solvents – N,N-Dimethylformamide | GC | NMT 880 ppm | Not detected |
| Residual solvents – Triisopropylsilane | GC | NMT 1000 ppm | Not detected |
| Bacterial endotoxins | Pharmacopeial method | < 10 EU/mg | Conforms |
| Total aerobic microbial count | Plate count | ≤ 1000 cfu/g | Conforms |
| Total yeast & mold count | Plate count | ≤ 200 cfu/g | Conforms |
| Assay (HPLC) | HPLC | 95.0 – 105.0% (anhydrous, salt-free basis) | 102.51% |
Storage & Handling
Storage Conditions:
- Store at −20°C or below in original sealed vial
- Protect from light and moisture
- Do not freeze–thaw repeatedly once reconstituted
- Recommended shelf life: 24 months when stored properly
General Laboratory Handling:
- Handle using standard laboratory safety practices
- Wear appropriate personal protective equipment (gloves, lab coat, safety glasses)
- Use in well-ventilated area or fume hood
- Avoid inhalation, ingestion, or skin/eye contact
- Wash hands thoroughly after handling
Reconstitution:
Reconstitution methods, diluents, and working concentrations must be determined by qualified researchers
according to their specific experimental protocols and institutional guidelines. Aventris does not provide
reconstitution instructions.
Source Batches:
- BPC-157 — Lot P260102-A015
- TB-500 Fragment — Lot P251226-A033
Peptide Sequences:
BPC-157 (15 AA):
GEPPPGKPADDAGLV-OH
TB-500 Fragment (7 AA):
Ac-LKKTETQ-OH
(Thymosin Beta-4 Fragment 17–23, N-terminal acetylated)
Molecular Identity:
- BPC-157: C₆₂H₉₈N₁₆O₂₂ — MW 1419.75 Da — CAS 137525-51-0
- TB-500 Fragment: C₃₈H₆₈N₁₀O₁₄ — MW 889.01 Da
Additional Documentation:
For questions about this batch or to request additional information, contact
[email protected]
Note: Any deviations from the above specification, if present, will be explicitly noted. In the absence of such notes, all parameters conform to the stated limits.